RNAi-directed downregulation of OsBADH2 results in aroma (2-acetyl-1-pyrroline) production in rice (Oryza sativa L.)

RNAi-directed downregulation of OsBADH2 results in aroma (2-acetyl-1-pyrroline) production in rice (Oryza sativa L.)

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Abstract Background Aromatic rice is popular worldwide because of its characteristic fragrance.Genetic studies and physical fine mapping Estudio de la carga externa de las tareas de baloncesto en función de las fases de juego (Study of external load in basketball tasks based on game phases) reveal that a candidate gene (fgr/OsBADH2) homologous to betaine aldehyde dehydrogenase is responsible for aroma metabolism in fragrant rice varieties, but the direct evidence demonstrating the functions of OsBADH2 is lacking.To elucidate the physiological roles of OsBADH2, sequencing approach and RNA interference (RNAi) technique were employed to analyze allelic variation and functions of OsBADH2 gene in aroma production.Semi-quantitative, real-time reverse transcription-polymerase chain reaction (RT-PCR), as well as gas chromatography-mass spectrometry (GC-MS) were conducted to determine the expression levels of OsBADH2 and the fragrant THE ROLE OF LEADERSHIP AGILITY AND ORGANIZATIONAL COMMITMENT TOWARD ORGANIZATIONAL READINESS FOR CHANGES IN PUBLIC ISLAMIC UNIVERSITIES OF CENTRAL JAVA IN CONDITIONS OF VUCA ERA compound in wild type and transgenic OsBADH2-RNAi repression lines, respectively.Results The results showed that multiple mutations identical to fgr allele occur in the 13 fragrant rice accessions across China; OsBADH2 is expressed constitutively, with less expression abundance in mature roots; the disrupted OsBADH2 by RNA interference leads to significantly increased 2-acetyl-1-pyrroline production.

Conclusion We have found that the altered expression levels of OsBADH2 gene influence aroma accumulation, and the prevalent aromatic allele probably has a single evolutionary origin.